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Hybridization

‘We are having problems with weak centromeric control probe signals, what could be the cause?’

This is likely to be caused by low humidity during the hybridization step. The FITC (green) fluorophore is more sensitive to these changes and can produce loss of or drop out of control probe signals. The optimal humidity levels for efficient probe hybridization are 65-70%, however we would recommend 95-100% humidity to minimize the risk of weak control signals.